Sino Biological/FAP Protein, Human, Recombinant (ECD, His Tag)/1/10464-H07H
商品编号：
10464-H07H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
FAP Protein, Human, Recombinant (ECD, His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >1200 pmol/min/μg
Protein Construction
A DNA sequence encoding the human FAP (Q12884-1) extracellular domain (Leu26-Asp760) was fused with the polyhistidie tag at the N-terminus.
Accession#
Q12884-1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
His
Molecule Mass
The recombinant human FAP consists of 751 amino acids and predicts a molecular mass of 87.2 kDa. As a result of glycosylation, rh FAP migrates as approximately 90 kDa band in SDS-PAGE under reducing conditions.
Formulation
Supplied as sterile 25mM Tris, 250mM NaCl, pH 8.2Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
FAP Protein, Human, Recombinant (ECD, His Tag): Images
FAP Protein, Human, Recombinant (ECD, His Tag): Synonyms
DPPIV Protein, Human; DPPIVA Protein, Human; FAPA Protein, Human; Fapalpha Protein, Human; Fibroblast Activation Protein alpha Protein, Human; SIMP Protein, Human
FAP Background Information
Seprase, also known as 17 kDa melanoma membrane-bound gelatinase , Fibroblast activation protein alpha, Integral membrane serine protease and FAP, is a single-pass type II membrane protein which belongs to thepeptidase S9B family. Seprase / FAP is found in cell surface lamellipodia, invadopodia and on shed vesicles. Seprase / FAP appears to act as a proteolytically active 17-kDa dimer, consisting of two 97-kDa subunits. It is a member of the group type II integral serine proteases, which includes dipeptidyl peptidase IV ( DPPIV / CD26 ) and related type II transmembrane prolyl serine peptidases, which exert their mechanisms of action on the cell surface. Seprase / FAP colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Seprase / FAP colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. DPP4 and seprase exhibit multiple functions due to their abilities to form complexes with each other and to interact with other membrane-associated molecules. In association with DPP4, Seprase / FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. Seprase / FAP has a dual function in tumour progression. The proteolytic activity of Seprase has been shown to promote cell invasiveness towards the ECM and also to support tumour growth and proliferation. Seprase / FAP may have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
fibroblast activation protein, alpha
Research Areas
- Serine Proteases and Regulators
- Cancer Drug Targets
References
- Mori,Y.et al., 2004, Oncology. 67(5-6):411-9.
- AertgeertsK., et al., 2005, J. Biol. Chem. 280:19441-19444.
- LiuT., et al., 2005, J. Proteome Res. 4:2070-2080.
- GhersiG., et al., 2006, Cancer Res. 66:4652-4661.
- O"Brien,P.et al., 2008,Biochim Biophys Acta. 1784(9):1130-45.
Human FAP Proteins
- FAP Protein, Human, Recombinant (His Tag), Biotinylated10464-H07H-B
Cynomolgus FAP Proteins
- FAP Protein, Cynomolgus, Recombinant (His Tag)90879-C07H
- Targeting cancer-associated fibroblasts by dual-responsive lipid-albumin nanoparticles to enhance drug perfusion for pancreatic tumor therapyAuthorYu, Q;Qiu, Y;Li, J;Tang, X;Wang, X;Cun, X;Xu, S;Liu, Y;Li, M;Zhang, Z;He, Q;Year2020JournalJ Control ReleaseApplicationcell-basedPubMed ID: 32112854FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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