Sino Biological/beta-Actin Loading Control Antibody, Mouse MAb/1/100166-MM10-免疫在线蚂蚁淘旗下平台

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商品详细Sino Biological/beta-Actin Loading Control Antibody, Mouse MAb/1/100166-MM10

Sino Biological/beta-Actin Loading Control Antibody, Mouse MAb/1/100166-MM10

商品编号： 100166-MM10

品牌： sinobiological

市场价： ¥0.00

美元价： 0.00

产地：美国(厂家直采)

公司：

产品分类：多肽合成

公司分类： peptide

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Description
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beta-Actin Loading Control Antibody, Mouse MAb General Information

Product name

beta-Actin Loading Control Antibody, Mouse MAb

Validated applications

WB,IP

Species reactivity

Reacts with: Human

Specificity

Human beta-Actin

Immunogen

A synthetic peptide corresponding to the N-terminus of the Human beta Actin / ACTB

Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the N-terminus of the Human beta Actin / ACTB. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Source

Monoclonal Mouse IgG2a Clone #10

Purification

Protein A

Formulation

0.2 μm filtered solution in PBS

Conjugate

Unconjugated

Form

Liquid

Shipping

This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

beta-Actin Loading Control Antibody, Mouse MAb Validated Applications

Application	Dilution
WB	1:2500-1:200000
IP	1-2 μL/mg of lysate

Please Note: Optimal concentrations/dilutions should be determined by the end user.

beta-Actin Loading Control Antibody, Mouse MAb Images

Human Beta-Actin Western blot (WB) 18676

Anti-beta-Actin mouse monoclonal antibody at 1:2500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: NIH3T3 Whole Cell Lysate

Lane C: RAW264.7 Whole Cell Lysate

Lane D: Mouse slpeen tissue lysate

Lane E: Mouse lung tissue lysate

Lane F: Mouse brain tissue lysate

Lane G: C6 whole cell lysate

Lane H: PC-12 whole cell lysate

Lane I: Rat liver tissue lysate

Lane J: Rat lung tissue lysate

Lane K: Rat brain tissue lysate

Lysates/proteins at 30 μg per lane.

Secondary

HRP Conjugated Goat anti-Mouse IgG (H+L) ,at 1:10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:43 kDa

Observed band size:44 kDa

Human Beta-Actin Western blot (WB) 18677

Anti-beta-Actin mouse monoclonal antibody at 1:2500 dilution

Lane A: HeLa Whole Cell Lysate

Lane B: JurkatWhole Cell Lysate

Lane C: MCF7 Whole Cell Lysate

Lane D: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:43 kDa

Observed band size:44 kDa

Human Beta-Actin Immunoprecipitation(IP) 18678

ACTB was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

0.5 µL anti-ACTB mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-ACTB mouse monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 42 kDa

Observed band size: 42 kDa

Human beta-Actin Western blot (WB) 19962

Anti-beta-Actin mouse monoclonal antibody at 1:10000, 1:50000, 1:100000, 1:200000 dilution

Lysates/proteins per lane

HepG2 (30 μg), Hela (5 μg), Raw246.7 (30 μg)

Secondary

Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 43 kDa

Observed band size: 44 kDa

beta-Actin Loading Control Antibody, Mouse MAb: Synonyms

Anti-BRWS1 Antibody; Anti-PS1TP5BP1 Antibody

beta-Actin Background Information

ACTB (Actin Beta) is a Protein Coding gene. Diseases associated with ACTB include Dystonia, Juvenile-Onset and Baraitser-Winter Syndrome 1. Among its related pathways are Salivary secretion and Development Slit-Robo signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding and RNA polymerase II proximal promoter sequence-specific DNA binding. An important paralog of this gene is ACTG1. TUBB, HPRT and ACTB were the most stably expressed genes for all analysis groups across meningioma and non-pathological meningeal tissue combined. ACTB, a significant upregulated gene in abdominal aortic aneurysm samples, could be regulated by CLIC4, which was significantly enriched in cell motions. Dystonia-deafness syndrome is a well-known clinical entity, with sensorineural deafness typically manifesting earlier than dystonia. ACTB p.Arg183Trp heterozygosity has been reported in six patients to cause combined infant-onset deafness and dystonia manifesting in adolescence or young adulthood.

Full Name

actin, beta

Anti-beta-Actin Antibody
【Rabbit】【Polyclonal】【WB】
109444-T32

CRISPR/Cas9-mediated LMP1 knockout inhibits Epstein-Barr virus infection and nasopharyngeal carcinoma cell growth
Author
Huo, H;Hu, G;
Year
2019
Journal
Infectious Agents and Cancer
Application
WB
FoldedExpand
Transcriptome Analysis Reveals That Abeliophyllum distichum Nakai Extract Inhibits RANKL-Mediated Osteoclastogenensis Mainly Through Suppressing Nfatc1 Expression
Author
Lee, K;Jang, YJ;Lee, H;Kim, E;Kim, Y;Yoo, TK;Hyun, TK;Park, JI;Yi, SJ;Kim, K;
Year
2020
Journal
Biology (Basel)
Application
WB
PubMed ID: 32781784FoldedExpand

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品牌介绍

北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。

公司介绍

品牌分类

Elisa试剂盒试剂蛋白质试剂抗体试剂 Gene Reagent

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官网：http://www.sinobiological.com/

Description

Related Products

Citations

Antibody Production Service

beta-Actin Loading Control Antibody, Mouse MAb General Information

beta-Actin Loading Control Antibody, Mouse MAb Validated Applications

beta-Actin Loading Control Antibody, Mouse MAb Images

beta-Actin Loading Control Antibody, Mouse MAb: Synonyms

beta-Actin Background Information

Citation List

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