Sino Biological/Beta-Tubulin Loading Control Antibody, Mouse MAb/1/100109-MM05T-免疫在线蚂蚁淘旗下平台

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商品详细Sino Biological/Beta-Tubulin Loading Control Antibody, Mouse MAb/1/100109-MM05T

Sino Biological/Beta-Tubulin Loading Control Antibody, Mouse MAb/1/100109-MM05T

商品编号： 100109-MM05T

品牌： sinobiological

市场价： ¥0.00

美元价： 0.00

产地：美国(厂家直采)

公司：

产品分类：多肽合成

公司分类： peptide

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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Beta-Tubulin Loading Control Antibody, Mouse MAb General Information

Product name

Beta-Tubulin Loading Control Antibody, Mouse MAb

Validated applications

WB,IHC-P,ICC/IF,IP

Species reactivity

Reacts with: Human

Specificity

Human Beta-Tubulin

Immunogen

A synthetic peptide corresponding to the N-terminus of the human beta-tubulin

Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the N-terminus of the human beta-tubulin. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Source

Monoclonal Mouse IgG2b Clone #05

Purification

Protein A

Formulation

0.2 μm filtered solution in PBS

Conjugate

Unconjugated

Form

Liquid

Shipping

This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Beta-Tubulin Loading Control Antibody, Mouse MAb Validated Applications

Application	Dilution
WB	1/1000-1/100000
IHC-P	1/2000-1/20000
ICC-IF	1/50-1/200
IP	IP:5-10 μL/mg of lysate

Please Note: Optimal concentrations/dilutions should be determined by the end user.

Beta-Tubulin Loading Control Antibody, Mouse MAb Images

Human Beta-Tubulin Immunohistochemistry(IHC) 18429

Immunochemical staining of human Tubulin in human breast carcinoma with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).

Human Beta-Tubulin Immunohistochemistry(IHC) 18430

Immunochemical staining of human Tubulin in human malignant melanoma with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).

Human Beta-Tubulin Immunohistochemistry(IHC) 18431

Immunochemical staining of human Tubulin in human prostate with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).

Human Beta-Tubulin Immunohistochemistry(IHC) 3002

Immunofluorescence staining of Human Beta-Tubulin in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human Beta-Tubulin monoclonal antibody at 4℃ overnight. Then cells were stained with the Alexa Fluor® 594-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor®488-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to cytoskeleton.

Human Beta-Tubulin Western blot (WB) 18425

Anti-Beta-Tubulin mouse monoclonal antibody at 1:10000, 1:20000, 1:50000, 1:100000 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lane C: Raw246.7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Rabbit Anti-Mouse IgG F(ab)2/HRPat 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:50 kDa

Observed band size:54 kDa

Human Beta-Tubulin Western blot (WB) 18426

Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution

Lane A: Rabbit heart tissue lysate

Lane B: Rabbit liver tissue lysate

Lane C: Rabbit spleen tissue lysate

Lane D: Rabbit lung tissue lysate

Lane E: Rabbit kidney tissue lysate

Lane F: Rabbit brain tissue lysate

Lane G: Rabbit muscle tissue lysate

Lane H: Rabbit stomach tissue lysate

Lane I: Rabbit small intestine tissue lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:50 kDa

Observed band size:54 kDa

Human Beta-Tubulin Western blot (WB) 18682

Anti-Beta-Tubulin mouse monoclonal antibody at 1:5000 dilution

Lane A: Rat heart tissue lysate

Lane B: Rat liver tissue lysate

Lane C: Rat spleen tissue lysate

Lane D: Rat lung tissue lysate

Lane E: Rat kidney tissue lysate

Lane F: Rat muscle tissue lysate

Lane G: Rat stomach tissue lysate

Lane H: Rat small intestine tissue lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:50 kDa

Observed band size:54 kDa

Human Beta-Tubulin Western blot (WB) 18681

Anti-Beta-Tubulin mouse monoclonal antibody at 1:5000 dilution

Lane A: Mouse heart tissue lysate

Lane B: Mouse liver tissue lysate

Lane C: Mouse spleen tissue lysate

Lane D: Mouse lung tissue lysate

Lane E: Mouse kidney tissue lysate

Lane F: Mouse brain tissue lysate

Lane G: Mouse muscle tissue lysate

Lane H: Mouse stomach tissue lysate

Lane I: Mouse pancreas tissue lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:50 kDa

Observed band size:54 kDa

Human Beta-Tubulin Western blot (WB) 18427

Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: K562 Whole Cell Lysate

Lane D: Hela Whole Cell Lysate

Lane E: RAW264.7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:50 kDa

Observed band size:54 kDa

Human Beta-Tubulin Western blot (WB) 18428

Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: Daudi Whole Cell Lysate

Lane C: MOLT-4 Whole Cell Lysate

Lane D: A549 Whole Cell Lysate

Lane E: 293T Whole Cell Lysate

Lane F: HelaS3 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:50 kDa

Observed band size:54 kDa

Beta-Tubulin Background Information

Beta-Tubulin is a subunit of tubulin. Tubulin is one of several members of a small family of globular proteins. It is the major constituent of microtubules. There are two most common members of the tubulin family: alpha-tubulin and beta-tubulin, and together their dimers form microtubules. The dimers of alpha- and beta-tubulin bind to GTP and assemble onto the (+) ends of microtubules while in the GTP-bound state. After the dimer is incorporated into the microtubule, the molecule of GTP bound to the beta -tubulin subunit eventually hydrolyzes into GDP through inter-dimer contacts along the microtubule protofilament. Beta-tubulin faces the plus end of the microtubule while alpha-tubulin faces the minus end. Dimers bound to GTP tend to assemble into microtubules, while dimers bound to GDP tend to fall apart. Loading controls are usually proteins that exhibit high-level, constitutive expression in the cell type or sample you are examining. This ensures constant expression levels. Thus “housekeeping genes” are frequently chosen for use as loading controls. It is also important that the protein chosen as a loading control has a different molecular weight than the protein of interest so that the bands are distinct and expression levels quantifiable. Popular loading control detection antibodies include anti-β-Actin monoclonal or polyclonal antibodies, anti-COX-4, anti-GAPDH, anti-Tubulin and anti-VDAC/Porin antibodies.

References

Williams RC Jr. et al., 1999. Anal Biochem. 275(2): 265-7.
Nogales E. et al., 1998. Nat Struct Biol. 5(6): 451-8.
Dutcher SK. 2001. Curr Opin Cell Biol. 13(1): 49-54.

ATG3, a Target of miR-431-5p, Promotes Proliferation and Invasion of Colon Cancer via Promoting Autophagy
Author
Huang, W;Zeng, C;Hu, S;Wang, L;Liu, J;
Year
2019
Journal
Cancer Manag Res
Application
WB
PubMed ID: 31849517FoldedExpand
β2 -adrenergic receptor signaling drives prostate cancer progression by targeting the Sonic hedgehog-Gli1 signaling activation
Author
Zhang, M;Wang, Q;Sun, X;Yin, Q;Chen, J;Xu, L;Xu, C;
Year
2020
Journal
Prostate
Application
WB
PubMed ID: 32894788FoldedExpand

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品牌介绍

北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。

公司介绍

品牌分类

Elisa试剂盒试剂蛋白质试剂抗体试剂 Gene Reagent

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QQ ：1570468124

手机：18915418616

官网：http://www.sinobiological.com/

Description

Related Products

Citations

Antibody Production Service

Beta-Tubulin Loading Control Antibody, Mouse MAb General Information

Beta-Tubulin Loading Control Antibody, Mouse MAb Validated Applications

Beta-Tubulin Loading Control Antibody, Mouse MAb Images

Beta-Tubulin Background Information

Citation List

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