Sino Biological/GAPDH Loading Control Antibody, Mouse Mab/1/100242-MM05
商品编号：
100242-MM05
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
GAPDH Loading Control Antibody, Mouse Mab General Information
Product name
GAPDH Loading Control Antibody, Mouse Mab
Validated applications
WB,IHC-P
Species reactivity
Reacts with: Human
Specificity
Human GAPDH
Immunogen
A synthetic peptide corresponding to the center regionof the human GAPDH
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the center region of the human GAPDH. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #05
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
GAPDH Loading Control Antibody, Mouse Mab Validated Applications
| Application | Dilution |
|---|---|
| WB | 1:500-1:2000 |
| IHC-P | 1:100-1:500 |
Please Note: Optimal concentrations/dilutions should be determined by the end user.
GAPDH Loading Control Antibody, Mouse Mab Images
Anti-GAPDH mouse monoclonal antibody at 1:500 dilution
Lane A: Jurkat Whole Cell Lysate
Lane B: Hela Whole Cell Lysate
Lane C: MCF7 Whole Cell Lysate
Lane D: MCF7 whole cell lysate
Lane E: PC-3 whole cell lysate
Lane F: Daudi whole cell lysate
Lane G: A549 whole cell lysate
Lane H: Rat heart tissue lysate
Lane I: Rat liver tissue lysate
Lane J: Rat spleen tissue lysate
Lane K: Rat lung tissue lysate
Lane L: Rat kidney tissue lysate
Lane M: Rat brain tissue lysate
Lysates/proteins at 30 μg per lane.
Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Predicted band size: kDa
Observed band size:38 kDa
Immunochemical staining of human GAPDH in human renal carcinoma with mouse monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human GAPDH in human cirrhosis with mouse monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.
GAPDH Loading Control Antibody, Mouse Mab: Synonyms
Anti-G3PD Antibody; Anti-GAPD Antibody; Anti-HEL-S-162eP Antibody
GAPDH Background Information
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer"s disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson"s disease, binds to GAPDH and displays neuroprotective actions.
Full Name
glyceraldehyde-3-phosphate dehydrogenase
Research Areas
- Adaptor Proteins
References
- Hara MR, et al. (2006) Neuroprotection by pharmacologic blockade of the GAPDH death cascade. PNA. 103 (10): 3887-9.
- Hara MR, et al. (2006) GAPDH as a sensor of NO stress.Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1762 (5): 502-9.
- Tarze A, et al. (2007) GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilizationGAPDH and apoptosis. Oncogene. 26: 2606-20.
- Yi MK, et al. (2000) Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function. Journal of Virology. 74 (14) : 6459-68.
- Difluoromethylornithine, a Decarboxylase 1 Inhibitor, Suppresses Hepatitis B Virus Replication by Reducing HBc Protein LevelsAuthorMao, B;Wang, Z;Pi, S;Long, Q;Chen, K;Cui, J;Huang, A;Hu, Y;Year2020JournalFront. Cell. Infect. Microbiol.ApplicationWBFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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