Sino Biological/重组抗EpCAM抗体，兔单克隆/1/10694-R028
商品编号：
10694-R028
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal General Information
Product name
Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal
Validated applications
WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IP
Species reactivity
Reacts with: Human
Specificity
Human EpCAMNo cross-reactivity in ELISA withHuman CD146 / MCAMHuman CD171 / N-CAML1 / L1CAMHuman OBCAM / OPCMLHuman TROP2 / TACSTD2
Immunogen
Recombinant Human EpCAM protein (Catalog#10694-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human EpCAM / TROP1 (rh EpCAM; Catalog#10694-H08H; NP_002345.1; Met 1-Lys 265).
Source
Monoclonal Rabbit IgG Clone #28
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal Validated Applications
| Application | Dilution |
|---|---|
| WB | 1:500-1:1000 |
| ELISA | 1:25000-1:50000 |
| IHC-P | 1:1000-1:5000 |
| ICC-IF | 1:250-1:5000 |
| FCM | 1:100-1:500 |
| IP | 0.2-1 μL/mg of lysate |
| ELISA(Det) | 1:5000-1:50000 |
Notes
| ELISA(Det)： | This antibody will detect Human EpCAM / TROP-1 / TACSTD1 in ELISA pair set (Catalog: # SEK10694). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10694-MM07). |
Please Note: Optimal concentrations/dilutions should be determined by the end user.
Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal Images
Immunofluorescence staining of Human EpCAM in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human EpCAM monoclonal antibody (1:500) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Immunochemical staining of human EpCAM in human mammary gland with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of alveolus epithelium.
Immunochemical staining of human EpCAM in human colon carcinoma with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of colonic gland epithelium.
Immunochemical staining of human EpCAM in human bladder carcinoma with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of transitional epithelium.
-R028-Purified-SKBR3.png)
Flow cytometric analysis of anti-EpCAM (CD326) reactivity on SKBR3 cells. SKBR3 cells were stained with anti-EpCAM (CD326) Monoclonal Ab (10694-R028), then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of viable cells.
Anti-EpCAM rabbit monoclonal antibody at 1:500 dilution
Lane A: MCF-7 Whole Cell Lysate
Lysates/proteins at 30 μg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Predicted band size:34 kDa
Observed band size:38 kDa
EpCAM was immunoprecipitated using:
Lane A:0.5 mg MCF-7 Whole Cell Lysate
Lane B:0.5 mg A431 Whole Cell Lysate
Lane C:0.5 mg HepG2 Whole Cell Lysate
Lane D:0.5 mg Caco-2 Whole Cell Lysate
0.5 µL anti-EpCAM rabbit monoclonal antibody and 15 μl of50 %Protein G agarose.
Primary antibody:
Anti-EpCAM rabbit monoclonal antibody,at 1:5000 dilution
Secondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution
Developed using the odssey technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal: Synonyms
Anti-DIAR5 Antibody; Anti-EGP-2 Antibody; Anti-EGP314 Antibody; Anti-EGP40 Antibody; Anti-ESA Antibody; Anti-HNPCC8 Antibody; Anti-KS1/4 Antibody; Anti-KSA Antibody; Anti-M4S1 Antibody; Anti-MIC18 Antibody; Anti-MK-1 Antibody; Anti-TACSTD1 Antibody; Anti-TROP-1 Antibody; Anti-TROP1 Antibody
EpCAM Background Information
Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â…transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
epithelial cell adhesion molecule
References
- Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.
- Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
- Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
- Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.
- HRP-conjugated plug-and-playable IgG-binding nanobodies as secondary antibody mimics in immunoassaysAuthorBae, Y;Jang, D;Eom, S;Park, T;Kang, S;Year2020JournalSensors and Actuators B: ChemicalFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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