Sino Biological/Recombinant Anti-CD34 Antibody, Rabbit Monoclonal/1/50589-R013
商品编号：
50589-R013
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Recombinant Anti-CD34 Antibody, Rabbit Monoclonal General Information
Product name
Recombinant Anti-CD34 Antibody, Rabbit Monoclonal
Validated applications
ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IP
Species reactivity
Reacts with: Mouse
Specificity
Mouse CD34
Immunogen
Recombinant Mouse CD34 Protein (Catalog#50589-M08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD34 (rM CD34; Catalog#50589-M08H; NP_001104529.1; Met 1-Thr 287).
Source
Monoclonal Rabbit IgG Clone #013
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Recombinant Anti-CD34 Antibody, Rabbit Monoclonal Validated Applications
| Application | Dilution |
|---|---|
| ELISA | 1:25000-1:50000 |
| IHC-P | 1:2000-1:10000 |
| ICC-IF | 1:100-1:500 |
| FCM | 1:100-1:500 |
| IP | 0.2-1 μL/mg of lysate |
| ELISA(Det) | 1:5000-1:50000 |
Notes
| ELISA(Det)： | This antibody will detect Mouse CD34 in ELISA pair set (Catalog: # SEK50589). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 50589-R028). |
Please Note: Optimal concentrations/dilutions should be determined by the end user.
Recombinant Anti-CD34 Antibody, Rabbit Monoclonal Images
Immunochemical Staining of mouse CD34 in mouse stomach with Rabbit Monoclonal Antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to vascular endothelial cells.
Immunochemical Staining of mouse CD34 in mouse kidney with Rabbit Monoclonal Antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to vascular endothelial cells.
Flow cytometric analysis of CD34 expression on M1 cells. Cells were stained with purified anti-Mouse CD34, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Mouse CD34 was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate
Lane B:0.5 mg 293T Whole Cell Lysate
0.5 µL anti-Mouse CD34 rabbit monoclonal antibody and 15 μl of50 %Protein G agarose.
Primary antibody:
Anti-Mouse CD34 rabbit monoclonal antibody,at 1:350 dilution
Secondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution
Developed using the odssey technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 130 kDa
Immunofluorescence staining of CD34 in M1 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD34 monoclonal antibody (1:300) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
Recombinant Anti-CD34 Antibody, Rabbit Monoclonal: Synonyms
Anti-AU040960 Antibody
CD34 Background Information
Cluster of Differentiation 34 (CD34) is a member of a family of single-pass transmembrane sialomucin proteins, and may function as a cell-cell adhesion factor. CD34 protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 monoclonal antibodies are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias.
Full Name
CD34 molecule
Research Areas
- Cardiac Stem Cell Markers
- Hemangioblast Markers
References
- Hogan CJ, et al. (2002) Differential long-term and multilineage engraftment potential from subfractions of human CD34+ cord blood cells transplanted into NOD/SCID mice. Proc Nat Acad Sci USA. 99 (1): 413-8.
- Nielsen JS,et al. (2009) CD34 is a key regulator of hematopoietic stem cell trafficking to bone marrow and mast cell progenitor trafficking in the periphery. Microcirculation. 16(6): 487-96.
- Mastrandrea F,et al. (2009) CD34+ hemopoietic precursor and stem cells traffic in peripheral blood of celiac patients is significantly increased but not directly related to epithelial damage severity. Eur Ann Allergy Clin Immunol. 40(3): 90-103.
- Pasquet S,et al. (2009) Long-term benefit of intracardiac delivery of autologous granulocyte-colony-stimulating factor-mobilized blood CD34+ cells containing cardiac progenitors on regional heart structure and function after myocardial infarct. Cytotherapy. 11(8): 1002-15.
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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