Anti-Myc tag Antibody, Mouse Monoclonal General Information
Anti-Myc tag Antibody, Mouse Monoclonal Validated Applications
| Application | Dilution |
|---|---|
| WB | 1/5000-1/10000 |
| IP | IP:1-5μL/mg of lysate |
Anti-Myc tag Antibody, Mouse Monoclonal Images
Anti-Myc Tag mouse monoclonal antibody at 1:5000 dilution.
Lane A: GST-myc(Recombinant protein) (5ng)
Lane B: GST-myc(Recombinant protein) (1ng)
Lane C: myc-GST(Recombinant protein) (100ng)
Lane D: myc-GST(Recombinant protein) (20ng)
Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution
Developed using the Odyssey technique.
Performed under reducing conditions.
Anti-Myc Tag mouse monoclonal antibody at 1:5000 dilution.
Lane A: HA-mFABP4-myc transfected 293 cell lysate (10ug)
Lane B: myc-ARG1-HA transfected 293 cell lysate (5ug)
Lane C: HA-ARG1-myc transfected 293 cell lysate (10ug)
Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution
Developed using the Odyssey technique.
Performed under reducing conditions.
MYC was immunoprecipitated using:
Lane A:0.5 mg HA-ARG1-mycWhole Cell Lysate
Lane B:0.5 mg myc-ARG1-HA Whole Cell Lysate
4 µL anti-MYC rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.
Primary antibody:
Anti-MYC rabbit polyclonal antibody,at 1:100 dilution
Secondary antibody:
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size :40 kDa
Myc tag Background Information
- Zhao X, et al. (2013) Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem 2013 581093.
- Hillman MC, et al. (2001) A comprehensive system for protein purification and biochemical analysis based on antibodies to c-myc peptide. Protein Expr Purif 23 (2): 359-368.
- Brothers SP, et al. (2003) Unexpected effects of epitope and chimeric tags on gonadotropin-releasing hormone receptors: Implications for understanding the molecular etiology of hypogonadotropic hypogonadism. J Clin Endocrinol Metab 88 (12): 6107-6112.
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