Sino-Biological/B7-H3-ELISA试剂盒，人/1/KIT11188
商品编号：
KIT11188
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
B7-H3 ELISA Kit, Human: General Information
Product name
B7-H3 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum,Urine
Sensitivity
16.20 pg/mL
Assay range
23.44-1500 pg/mL
Specificity
Recognizes both recombinant and natural Human B7-H3
Interference
Preparations of human B7-1, B7-2 and PD-L2at 50 ng/mL in a mid-range recombinant human B7H3 control were assayed for interference. No significant interference was observed.
Precision
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 264.28 | 490.13 | 932.43 |
| SD | 6.32 | 12.00 | 25.22 |
| CV(%) | 2.4% | 2.4% | 2.7% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 272.45 | 547.73 | 1,022.38 |
| SD | 13.81 | 23.71 | 32.98 |
| CV(%) | 5.1% | 4.3% | 3.2% |
Recovery
The recovery of Human CD276 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 86 |
| Range |
| 80 -90% |
| Sample |
| urine (n=3) |
| Average % Recovery |
| 107 |
| Range |
| 106 -109% |
Linearity
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 109% |
| 1:4 | 130% |
| 1:8 | 123% |
| 1:16 | 107% |
Urine
| Recovery of detected | |
|---|---|
| 1:2 | 92% |
| 1:4 | 108% |
| 1:8 | 118% |
| 1:16 | 99% |
Materials provided
1. 96 well microplate coated with Capture Antibody2. Detection Antibody conjugated to HRP3. Standards4. Wash Buffer Concentrate5. Dilution Buffer Concentrate6. Color Reagent A7. Color Reagent B8. Stop Solution
Product overview
This B7-H3 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human B7-H3 protein in Serum,Urine . It contains recombinant Human B7-H3, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃Opened/Reconstituted Reagents: Please refer to CoA
B7-H3 ELISA Kit, Human: Images
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human B7H3. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
B7-H3 ELISA Kit, Human: Synonyms
4Ig-B7-H3 ELISA Kit, Human; B7-H3 ELISA Kit, Human; B7H3 ELISA Kit, Human; B7RP-2 ELISA Kit, Human
B7-H3 Background Information
B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.
- Cancer Immunotherapy
- Co-inhibitory Immune Checkpoint Targets
- Immune Checkpoint
- Immune Checkpoint Detection: Antibodies
- Immune Checkpoint Detection: ELISA Antibodies
- Immune Checkpoint Detection: FCM Antibodies
- Immune Checkpoint Detection: ICC Antibodies
- Immune Checkpoint Detection: IP Antibodies
- Immune Checkpoint Detection: WB Antibodies
- Immune Checkpoint Targets
- Immunotherapy
- Targeted Therapy
Full Name
CD276 molecule
Research Areas
- Cancer Drug Targets
References
- Suh WK, et al. (2004) The immune regulatory protein B7-H3 promotes osteoblast differentiation and bone mineralization. Proc Natl Acad Sci U S A. 101(35): 12969-73.
- Waschbisch A, et al. (2008) Human muscle cells express the costimulatory molecule B7-H3, which modulates muscle-immune interactions. Arthritis Rheum. 58(11): 3600-8.
- Loos M, et al. (2010) B7-h3 and its role in antitumor immunity. Clin Dev Immunol. 2010: 683875.
- Zang X, et al. (2010) Tumor associated endothelial expression of B7-H3 predicts survival in ovarian carcinomas. Mod Pathol. 23(8): 1104-12.
- Sun J, et al. (2010) Clinical significance and regulation of the costimulatory molecule B7-H3 in human colorectal carcinoma. Cancer Immunol Immunother. 59(8): 1163-71.
- MicroRNA-199a Inhibits Cell Proliferation, Migration, and Invasion and Activates AKT/mTOR Signaling Pathway by Targeting B7-H3 in Cervical CancerAuthorYang, X;Feng, KX;Li, H;Wang, L;Xia, H;Year2020JournalTechnol. Cancer Res. Treat.ApplicationELISAPubMed ID: 32856542FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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