Sino Biological/CXCL5 ELISA试剂盒，人/1/KIT10889
商品编号：
KIT10889
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
CXCL5 ELISA Kit, Human: General Information
Product name
CXCL5 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum,Cell supernatant,Plasma
Sensitivity
1.44 pg/mL
Assay range
4.69-300 pg/mL
Specificity
Recognizes both recombinant and natural Human CXCL5
Interference
Preparations ofthe factors listed below at 50 ng/mL in a mid-range recombinant human CXCL5 control were assayed for interference. No significant interference was observed.
Precision
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 61.94 | 99.46 | 191.27 |
| SD | 2.65 | 5.03 | 7.23 |
| CV(%) | 4.3% | 5.1% | 3.8% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 59.84 | 102.41 | 199.19 |
| SD | 1.48 | 3.62 | 4.33 |
| CV(%) | 2.5% | 3.5% | 2.2% |
Recovery
The recovery of Human CXCL5 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 110 |
| Range |
| 105-119% |
| Sample |
| supernatant (n=3) |
| Average % Recovery |
| 121 |
| Range |
| 112-129% |
| Sample |
| plasma (n=3) |
| Average % Recovery |
| 106 |
| Range |
| 103-112% |
Linearity
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 110% |
| 1:4 | 103% |
| 1:8 | 97% |
| 1:16 | 72% |
Supernatant
| Recovery of detected | |
|---|---|
| 1:2 | 99% |
| 1:4 | 97% |
| 1:8 | 97% |
| 1:16 | 90% |
Plasma
| Recovery of detected | |
|---|---|
| 1:2 | 104% |
| 1:4 | 105% |
| 1:8 | 95% |
| 1:16 | 84% |
Materials provided
1. 96 well microplate coated with Capture Antibody2. Detection Antibody conjugated to HRP3. Standards4. Wash Buffer Concentrate5. Dilution Buffer Concentrate6. Color Reagent A7. Color Reagent B8. Stop Solution
Product overview
This CXCL5 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL5 protein in Serum,Cell supernatant,Plasma . It contains recombinant Human CXCL5, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃Opened/Reconstituted Reagents: Please refer to CoA
CXCL5 ELISA Kit, Human: Images
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CXCL5. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
CXCL5 ELISA Kit, Human: Synonyms
ENA-78 ELISA Kit, Human; SCYB5 ELISA Kit, Human
CXCL5 Background Information
CXCL5 is a small cytokine belonging to the CXC chemokine family. CXC chemokines are particularly significant for leukocyte infiltration in inflammatory diseases. CXCL5 is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. It also can be detected in eosinophils, and can be inhibited with the type II interferon. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis. It stimulates the chemotaxis of neutrophils possesses angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2.
Full Name
chemokine (C-X-C motif) ligand 5
Related Pathways
IL17 signaling pathway
References
- Dawes JM, et al. (2011) CXCL5 Mediates UVB Irradiation-Induced Pain. Sci Transl Med. 3(90): 90ra60.
- O"Donovan N, et al. (1999) Physical mapping of the CXC chemokine locus on human chromosome 4. Cytogenet. Cell Genet. 84(1-2):39-42.
- Persson T, et al. (2003) Expression of the neutrophil-activating CXC chemokine ENA-78/CXCL5 by human eosinophils. Clin Exp Allergy. 33(4):531-7.
- Schwann cells promote lung cancer proliferation by promoting the M2 polarization of macrophagesAuthorZhou, Y;Li, J;Han, B;Zhong, R;Zhong, H;Year2020JournalCellular ImmunologyApplicationELISAFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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